Trichomonas vaginalis Infection of SiHa Cells

T. vaginalis T106 was provided by Prof. J. K. Alderete (University of Texas, Health Science Center, TX, USA) and cultured in TYM medium consisting of 20% (w/v) trypticase peptone, 10% (w/v) yeast extract, 5% (w/v) Moltose monodydrate, 1% (w/v) L-cystein hydrochloride, 1% (w/v) ascorbic acid, 1% (w/v) K₂HPO₄, 1% (w/v) KH₂PO₄ (pH 6.2) with 10% heat-inactivated horse serum (Sigma-Aldrich, St. Louis, MO, USA) and penicillin-streptomycin (Gibco BRL) and incubated under 5% CO₂ at 37 °C SiHa cells were infected with live T. vaginalis at multiplicities of infection (MOI) of 1 in the mixed-medium (DMEM without Antibiotic: TYM = 2:1) as previously described [25 (link)].