Western Blot Antibody Analysis Protocol

Western blot analysis was performed as described previously [9 (link)]. The antibody information used in the experiment is shown below: p38 (#8690S, 1:1000; Cell Signaling Technology, Danvers, MA, USA), Bcl-2 (AF6139, 1:1000; Affinity Bioscience), Bax (#2772, 1:1000; Cell Signaling Technology), AMPKα (#5823, 1:1000; Cell Signaling Technology), p-AMPKα (#11818, 1:1000; Cell Signaling Technology), SIRT1 (#2493, 1:1000; Cell Signaling Technology), PGC1α (AF5395, 1:1000; Affinity), TLR4 (AF7017, 1:1000; Affinity), p65 (#8242, 1:1000; Cell Signaling Technology), p-p65 (#3033, 1:1000; Cell Signaling Technology), p-IKBα (#2859, 1:1000; Cell Signaling Technology), β-actin (AC038, 1:1000; ABclonal, Woburn, MA, USA), and horseradish peroxidase (A0208, 1:1000; Beyotime, Shanghai, China). Differences in protein transfer efficiency between blots were normalized based on the quantification of β-actin.