Anesthesia was performed by using 2% isoflurane via inhalation. Cerebral ischemia was induced using the intraluminal suture middle cerebral artery occlusion (MCAO) model [9 (link)]. Nineteen to 21 mm 3–0 surgical nylon filament was introduced from the external carotid artery lumen into the internal carotid artery to block the origin of the right MCA. The animals were kept under anesthesia for only 15 min for the surgical procedure. Temperature was maintained at 36.5–37.5°C using a controlled heating system. After 3 h of occlusion, the animals received either candesartan (Astra-Zeneca) 1 mg/kg or an equal volume of saline (1 mL/kg) intravenously, and the animals were returned to their cages. The selected candesartan dose was previously shown to be neurovascular protective [5 (link), 6 (link)]. Animals were euthanized at either 24 h or 7 days after stroke.
Protocol detail
Candesartan as Neuroprotective Stroke Therapy
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Anesthesia
Animals
Block
Candesartan
Cerebral ischemia
External carotid artery
Filament
Inhalation
Internal carotid artery
Isoflurane
Middle cerebral artery occlusion
Nylon
Occlusion
Saline
Stroke
Surgical procedure
Suture
Corresponding Organization :
Other organizations : Augusta University, University of Georgia, Charlie Norwood VA Medical Center
Top 5 similar protocols
Protocol cited in 5 other protocols
Variable analysis
independent variables
- Candesartan (1 mg/kg) administration
- Ischemic brain injury at 24 h and 7 days after stroke
- Anesthesia using 2% isoflurane via inhalation
- Cerebral ischemia induced using the intraluminal suture middle cerebral artery occlusion (MCAO) model
- Duration of anesthesia for the surgical procedure (15 min)
- Temperature maintained at 36.5-37.5°C using a controlled heating system
- Positive control: Candesartan (1 mg/kg) administration
- Negative control: Saline (1 mL/kg) administration
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!