Release studies were performed by incubation of lentivirus in serum containing media at 37 °C in 293T cells, with samples (100 μL) collected via replacement at the indicated time points and stored at −80 °C until the sample concentration was determined. Viral particle concentrations were determined using HIV-1 p24 Antigen ELISA (ZeptoMetrix, Franklin, MA, USA) [24 (link)].
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