Cell Lines Characterization and Maintenance

HEK-293
cells were obtained
from the American Type Culture Collection (ATCC, Manassas, VA). HEK-293
derivative lines 293pTP and 293 RAPA cells were previously characterized.^{55 (link),56 (link)} Mouse mesenchymal stem cell (MSC) line iMEFs was previously characterized.^{57 (link),58 (link)} All cell lines were maintained in DMEM supplemented with 10% fetal
bovine serum (FBS, Gemini Bio-Products, West Sacramento, CA), containing
100 U/mL penicillin and 100 μg/mL streptomycin, at 37 °C
in 5% CO₂ as described.^{21 (link),59 (link),60 (link)} All restriction endonuclease enzymes were purchased
from New England Biolabs (NEB, Ipswich, MA). Blasticidin S was purchased
from InvivoGen (San Diego, CA). Unless indicated otherwise, other
reagents were purchased from Sigma-Aldrich (St. Louis, MO) or Thermo
Fisher Scientific (Waltham, MA).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Wang X., Yuan C., Huang B., Fan J., Feng Y., Li A.J., Zhang B., Lei Y., Ye Z., Zhao L., Cao D., Yang L., Wu D., Chen X., Liu B., Wagstaff W., He F., Wu X., Luo H., Zhang J., Zhang M., Haydon R.C., Luu H.H., Lee M.J., Moriatis Wolf J., Huang A., He T.C, & Zeng Z. (2019). Developing a Versatile Shotgun Cloning Strategy for Single-Vector-Based Multiplex Expression of Short Interfering RNAs (siRNAs) in Mammalian Cells. ACS Synthetic Biology, 8(9), 2092-2105.

Publication 2019

HEK-293cells HEK-293 FBS Blasticidin S

Blasticidin s Cell lines Cells Enzymes Mesenchymal stem cell Mouse Penicillin Restriction endonuclease Serum Streptomycin

Corresponding Organization : University of Chicago Medical Center

Other organizations : China Three Gorges University, Second Affiliated Hospital of Nanchang University, Nanchang University, Lanzhou University, Lanzhou University Second Hospital, Soochow University, Second Affiliated Hospital of Soochow University, First People's Hospital of Chongqing, Qingdao University, Affiliated Hospital of Qingdao University, Southwest University, West China Hospital of Sichuan University, Sichuan University, First Affiliated Hospital of Guangzhou University of Chinese Medicine

Top 5 similar protocols

Protocol cited in 2 other protocols

Variable analysis

independent variables

HEK-293 cells
HEK-293 derivative lines 293pTP and 293 RAPA cells
Mouse mesenchymal stem cell (MSC) line iMEFs

dependent variables

Not explicitly mentioned

control variables

All cell lines were maintained in DMEM supplemented with 10% fetal bovine serum (FBS, Gemini Bio-Products, West Sacramento, CA), containing 100 U/mL penicillin and 100 μg/mL streptomycin, at 37 °C in 5% CO2

controls

Positive controls: Not mentioned
Negative controls: Not mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!