The M. xanthus strains used in this study were DK10416 (ΔpilB) (26 (link)), YZ603 (ΔdifE) (50 (link)), YZ1674 (ΔpilB att::MxpilB) (51 (link)), and YZ2232 (ΔpilB att::MC3pilB) (31 (link), 63 ). Unless stated otherwise, they were maintained and grown on Casitone-yeast extract (CYE) agar plates or media at 32°C. Conditions and procedures for the expression and purification of the CtPilB protein were as previously described (31 (link)).
All stock solutions of quercetin used in this study were prepared by dissolving quercetin hydrate (ACROS Organics) in dimethyl sulfoxide (DMSO; Fisher Biotech). Unless otherwise stated, 10× stocks were prepared for each concentration of quercetin in DMSO before their use in experiments. Controls without quercetin had the same concentration of DMSO as those with quercetin. The Selleckchem kinase library L1200 used for the HTS had been reformulated to be 1 mM stocks in DMSO.
GraphPad Prism v 7.04 was used for curve fitting and data analysis. Student’s t test was used for statistical analysis.
All stock solutions of quercetin used in this study were prepared by dissolving quercetin hydrate (ACROS Organics) in dimethyl sulfoxide (DMSO; Fisher Biotech). Unless otherwise stated, 10× stocks were prepared for each concentration of quercetin in DMSO before their use in experiments. Controls without quercetin had the same concentration of DMSO as those with quercetin. The Selleckchem kinase library L1200 used for the HTS had been reformulated to be 1 mM stocks in DMSO.
GraphPad Prism v 7.04 was used for curve fitting and data analysis. Student’s t test was used for statistical analysis.
Free full text: Click here
