Western Blot Protein Detection

The general procedure for Western blotting was performed as reported previously [22 (link),23 (link)]. The following antibodies were used: anti-myc (sc-40; 1:1000 dilution, Santa Cruz Biotechnology, Dallas, TX, USA); alpha-tubulin (CP06; 1:1000 dilution, Merck Millipore, Darmstadt, Germany); anti-mouse IgG (NA931; 1:5000 dilution, GE Healthcare, Chicago, IL, USA); and anti-rabbit IgG (NA934; 1:5000 dilution, GE Healthcare, Chicago, IL, USA).

Free full text: Click here

Dozen A., Shozu K., Shinkai N., Ikawa N., Aoyama R., Machino H., Asada K., Yoshida H., Kato T., Hamamoto R., Kaneko S, & Komatsu M. (2022). Tumor Suppressive Role of the PRELP Gene in Ovarian Clear Cell Carcinoma. Journal of Personalized Medicine, 12(12), 1999.

Publication 2022

anti-myc (sc-40; anti-mouse IgG (NA931; anti-rabbit IgG (NA934;

Alpha tubulin Anti igg Antibodies Dilution Mouse Rabbit

Corresponding Organization : RIKEN Center for Advanced Intelligence Project

Other organizations : Keio University Hospital, University of Toyama, Tokyo Medical and Dental University, Showa University, National Cancer Center Hospital East

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Myc protein expression levels
Alpha-tubulin protein expression levels

control variables

Concentrations of primary and secondary antibodies used for Western blotting

controls

Positive control: None explicitly mentioned
Negative control: None explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!