Evaluating AU's Effects on Cell Viability

Rat adrenal pheochromocytoma cells (PC12) and human rheumatoid fibroblast-like synoviocytes (HFLS) were obtained from iCell Bioscience Inc. and JENNIO Biological Technology, respectively. PC12 cells were cultured in 1640 basal medium containing 10% fetal bovine serum (FBS) and 1% Penicillin-Streptomycin, while HFLS cells were cultured in DMEM basal medium with the same supplements. The cells were incubated at 37°C in an atmosphere containing 5% CO2. PC12 cells were seeded at a density of 2x10⁴ cells/well and incubated for 24 hours before exposure to AU. Different concentrations of AU (ranging from 0 to 160 μM) were then added to the wells and incubated for an additional 24 hours. MTT solution (10 μM) was added to each well and incubated for a further 4 hours. The medium was then removed and DMSO (200 μl) was added to dissolve the formazan crystals formed by the viable cells. The absorbance at 490 nm was measured using a microplate reader. To evaluate the effect of AU on cell proliferation ability, different concentrations of AU ranging from 0 to 5 mM were prepared and tested.