In vivo electroporation was used to deliver plasmids into the subretinal space, as previously described (Matsuda and Cepko, 2007 (link); updated electroporation protocol, described in detail in Wang et al., 2014 (link)). Camk2a-Cre pups were injected with a Cre-dependent reporter plasmid (at 1 µg/µl concentration, 0.3–0.5 µl of solution/injection area with two distinct electroporation patches per eye) expressing mCherry (pAAV-flexTC66T; Miyamichi et al., 2013 (link)) on postnatal day (p)0. Injections were performed using a pulled angled glass pipette controlled by a Femtojet Express pressure injector (Eppendorf, E5242956003) into the right eye. A short electric pulse was given to the animals right after the injections, to electroporate the construct. After weaning, mice were killed, and the retinas were processed for cryosections, as described above.
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