Pretreated cells were planted in 6 well plates for about 24 h. The middle of the cells in each plate was scratched using a sterilized 200 μL pipette tip, followed by washing with 1 × PBS and then adding 2 ml DMEM containing 2% FBS and 1 mM thymidine (Sigma-Aldrich, USA) into each well. Monitoring the scratches width at 0 and 24 h, respectively.
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