Cultivation of Helicobacter pylori Strains

All experiments reported in this paper were done on the LSH100 strain of H. pylori, a derivative of the human clinical isolate G27 (38 (link), 57 (link)) and its isogenic mutant with a straight rod shape, LSH100 Δcsd6 (26 (link), 28 (link)). The culture procedure was identical to that used by Martinez et al. (28 (link)) and is described briefly here. Frozen aliquots of bacteria were grown on Brucella agar plates with 5% horse blood (BD Biosciences) for 2 days, after which, they were restreaked onto new plates. After 2 to 5 days, the grown cells were transferred into BB10 containing Brucella broth (BD Biosciences) with 10% heat-inactivated fetal bovine serum (GIBCO) and left to grow overnight under constant agitation. Both cultures in agar plate and BB10 were kept in an incubator at 37°C under microaerophilic conditions (6 to 16% oxygen and 2 to 10% carbon dioxide; BD Biosciences GasPak EZ Campy Container System Sachets).

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Constantino M.A., Jabbarzadeh M., Fu H.C, & Bansil R. (2016). Helical and rod-shaped bacteria swim in helical trajectories with little additional propulsion from helical shape. Science Advances, 2(11), e1601661.

Publication 2016

Brucella agar plates horse blood Brucella broth

Agar Bacteria Blood Brucella Carbon 10 Carbon dioxide Cells Fetal bovine serum Frozen H pylori Horse Human Oxygen 16 Strain

Corresponding Organization : University of Utah

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Variable analysis

independent variables

Strain: LSH100 (a derivative of the human clinical isolate G27) and its isogenic mutant with a straight rod shape, LSH100 Δcsd6

dependent variables

Bacterial growth and morphology

control variables

Culture procedure: Identical to that used by Martinez et al.
Culture conditions: 37°C under microaerophilic conditions (6 to 16% oxygen and 2 to 10% carbon dioxide)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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