Genetically Modified Mouse Models

CD11c-cre Il27p28^fl/fl mice (Lee et al., 2015 (link)) and Foxp3^creIl27ra^fl/fl mice (Do et al., 2017 (link)) were described previously. In addition, CD4-cre (Lee et al., 2001 (link)), Lysozyme^cre (Clausen et al., 1999 (link)), Vil-cre mice (Madison et al., 2002 (link)), and CD45.1⁺ B6 mice were purchased from the Jackson Laboratory. All mice were bred and housed under specific pathogen–free conditions. 8–12-wk-old mice of both sexes were used as young mice, 6–7-mo-old mice were used as aged mice, and only WT littermates of the same gender served as controls in each experiment. All mice were maintained and handled in accordance with the Institutional Animal Care and Use Guidelines of University of California, San Diego and National Institutes of Health Guidelines for the Care and Use of Laboratory Animals and the Animal Research: Reporting In Vivo Experiments guidelines.