Culturing Classical CTCL Cell Lines

Experiments were performed on six classical CTCL cell lines, including four cALCL: Mac1, Mac2A, Mac2B (DSMZ), and FEPD (Prof. G. Delsol, Toulouse, France); one T-MF: MyLa (Dr K. Kaltoft, Aarhus, Denmark); and one SS: Hut78 (ATCC). Furthermore, we included two SS cell lines newly developed in our laboratory: L1 and L2 [47 (link)]. A human T-cell leukemia cell line, 1301 (Sigma-Aldrich), was used as a positive control for the amplification of the hTERT RT domain splicing variants. The cells were cultured in an RPMI 1640 media (Gibco, Waltham, MA, USA) supplemented with 1% penicillin–streptomycin (Gibco) and 10% fetal bovine serum (Eurobio, Les Ulis, France), except for the L1 and L2 cell lines, which were cultured as previously described [47 (link)]. All cell lines were incubated at 37 °C with 5% CO₂ and regularly tested for mycoplasma contamination.