Intravital microscopy was performed on an Olympus FV1000 confocal-multiphoton imaging system using a XLUMPLFLN 20x water immersion objective (NA 1.0; Olympus America) with 2x digital zoom. Images were scanned sequentially using 405-nm, 473-nm, 559-nm, and 635-nm diode lasers with a DM405/473/559/635-nm dichroic beam splitter; emitted light was collected using combinations of beam splitters (SDM473, SDM560, and/or SDM 640) and emission filters BA430-455, BA490-540, BA575-620, and BA655-755 (all Olympus America).
Dorsal window chamber imaging was performed following previously described procedures (16 (link)); briefly, 2 million HT1080-membrane-mApple cells in 50 μl PBS were injected under the fascia of nu/nu mice (Cox7, MGH) 30 min after surgical chamber implantation and imaged two weeks later.
Dorsal window chamber imaging was performed following previously described procedures (16 (link)); briefly, 2 million HT1080-membrane-mApple cells in 50 μl PBS were injected under the fascia of nu/nu mice (Cox7, MGH) 30 min after surgical chamber implantation and imaged two weeks later.
