Quantitative Analysis of MDPV Analogs

MDPV and MDPV-d8 were obtained from Cayman Chemical Company (Ann Arbor, MI, USA) and Cerilliant (Round Rock, TX, USA). 3,4-Catechol-PV and 4-OH-3-MeO-PV were synthesized and purified by the Drug Design and Synthesis Section of the National Institute on Drug Abuse (NIDA) Intramural Research Program (IRP), Baltimore, MD, USA (Anizan et al. 2014 (link)). Formic acid, methanol, LC-MS grade acetonitrile (ACN) and water, ethylenediaminetetraacetic acid (EDTA) and sodium metabisulfite (SMBS) were acquired from Fisher Scientific (Fair Lawn, NJ, USA). 4-Methylcatechol and sodium hydroxide were purchased from Sigma (St Louis, MO, USA) and JT Baker (Phillipsburg, NJ, USA), respectively. β-glucuronidase from Red Abalone was obtained from Kura Biotec (Culver City, CA, USA). Water for EDTA, 4-Methylcatechol and SMBS solution preparation was purified-in-house by an ELGA Purelab Ultra Analytic purifier (Siemens Water Technologies, Lowell, MA, USA).