Generation and Characterization of Genetically Modified Mice

Wild-type C57BL/6, Ccr2^RFP reporter (B6.129(Cg)-Ccr2^tm2.1Ifc/J), Cx3cr1^GFP reporter (B6.129P-Cx3cr1^tm1Litt/J), CD45.1 (B6.SJL-Ptprc^a Pepc^b/BoyJ), Raptor ^fl/fl (B6.Cg-Rptor^tm1.1Dmsa/J), ubiquitin-ERT2-cre (B6.Cg-Tg(UBC-cre/ERT2)1Ejb/1J), Mx1-Cre (B6.Cg-Tg(Mx1-cre)1Cgn/J), and LysM-Cre (B6.129P2-Lyz2^tm1(cre)Ifo/J) mice were obtained from Jackson Laboratories. Femurs from Myc^GFP mice (B6;129-Myc^tm1Slek/J) were also obtained from Jackson Laboratories. Ccr2^RFP/+ Cx3cr1^GFP/+ (dual reporter) mice, Cre/ERT2 Raptor ^fl/fl mice (Raptor iKO), Mx1-Cre Raptor ^fl/fl mice and LysMCre Raptor ^fl/fl mice were generated as described (19 (link), 26 (link), 56 (link)). Tsc2 ^fl/fl (Tsc2^tm1.1Mjg/J) mice were kindly provided by Dr. Michael Gambello (57 (link)) and bred with Mx1-Cre mice to generate Tsc2 KO mice. Male and female mice age between 6 to 8 weeks were used with matched littermate controls.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Lee P.Y., Sykes D.B., Ameri S., Kalaitzidis D., Charles J.F., Nelson-Maney N., Wei K., Cunin P., Morris A., Cardona A.E., Root D.E., Scadden D.T, & Nigrovic P.A. (2017). The metabolic regulator mTORC1 controls terminal myeloid differentiation. Science immunology, 2(11), eaam6641.

Publication 2017

B6.129(Cg)-Ccr2tm2.1Ifc/J), B6.Cg-Tg(Mx1-cre)1Cgn/J LysM-Cre (B6.129P2-Lyz2tm1(cre)Ifo/J) mice

Ert2 Female Femurs Male Mice Raptor Tsc2 Ubiquitin

Corresponding Organization : Brigham and Women's Hospital

Other organizations : Massachusetts General Hospital, The University of Texas at San Antonio, Broad Institute, Massachusetts Institute of Technology

Top 5 similar protocols

Variable analysis

independent variables

Genetic modifications to mice: Ccr2^RFP reporter, Cx3cr1^GFP reporter, CD45.1, Raptor ^fl/fl, ubiquitin-ERT2-cre, Mx1-Cre, LysM-Cre, Myc^GFP, Ccr2^RFP/+ Cx3cr1^GFP/+, Cre/ERT2 Raptor ^fl/fl, Mx1-Cre Raptor ^fl/fl, LysMCre Raptor ^fl/fl, Tsc2 ^fl/fl, Mx1-Cre

dependent variables

Not explicitly mentioned

control variables

Wild-type C57BL/6 mice
Matched littermate controls for genetically modified mice

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!