Multiplex Viral Detection from Serum

Nucleic acids were extracted from serum samples using the EZ1 Virus Mini Kit v2 and an EZ1 advanced XL Biorobot workstation (Qiagen). Detection of viral genomes was performed using a one-step TaqMan real time RT-PCR procedure and the kit SuperScript III Platinum One-Step Quantitative RT-PCR System with ROX (Life Technologies) for the CHIKV and the dengue virus (DENV), as previously described [21] (link), [22] (link), [23] (link). In addition, plasma samples were tested for the presence of Plasmodium falciparum genomes using a previously reported real time PCR procedure targeting the P. falciparum aquaglyceroporin gene [21] (link), [22] (link), [23] (link) and the Platinum Quantitative PCR SuperMix-UDG (Life Technologies).

Free full text: Click here

Moyen N., Thiberville S.D., Pastorino B., Nougairede A., Thirion L., Mombouli J.V., Dimi Y., Leparc-Goffart I., Capobianchi M.R., Lepfoundzou A.D, & de Lamballerie X. (2014). First Reported Chikungunya Fever Outbreak in the Republic of Congo, 2011. PLoS ONE, 9(12), e115938.

Publication 2014

EZ1 Virus Mini Kit v2 SuperScript III Platinum One-Step Quantitative RT-PCR System with ROX Platinum Quantitative PCR SuperMix-UDG

Aquaglyceroporin Dengue virus Gene Genomes Nucleic acids Plasma Plasmodium falciparum Platinum Real time pcr Rt pcr Serum Viral genomes Virus

Corresponding Organization :

Other organizations : Institut de Recherche pour le Développement, Aix-Marseille Université, Laboratoire National de Santé Publique du Congo, National Institutes of Health

Top 5 similar protocols

Variable analysis

independent variables

Method of nucleic acid extraction: EZ1 Virus Mini Kit v2 and EZ1 advanced XL Biorobot workstation (Qiagen)
Detection method for viral genomes: One-step TaqMan real time RT-PCR procedure using the SuperScript III Platinum One-Step Quantitative RT-PCR System with ROX (Life Technologies)
Detection method for Plasmodium falciparum genomes: Real time PCR procedure targeting the P. falciparum aquaglyceroporin gene using the Platinum Quantitative PCR SuperMix-UDG (Life Technologies)

dependent variables

Presence/detection of CHIKV viral genomes
Presence/detection of dengue virus (DENV) viral genomes
Presence/detection of Plasmodium falciparum genomes

control variables

Serum samples

positive controls

None specified

negative controls

None specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!