Protein Expression Analysis of Vascular Cells

Cell extracts were prepared [9 (link), 32 (link)], electrophoresed by sodium dodecyl sulfate– polyacrylamide gel electrophoresis (SDS-PAGE), transferred to nitrocellulose, and probed with anti-human PlexinD (R&D Systems), anti- αTubulin (EMD Millipore), anti-JAGGED1( Cell Signaling Technology, Danvers, MA), anti-human Calponin (Dako, Carpinteria, CA), anti-human CD31 (Santa Cruz Biotechnology, Inc., Dallas, Texas), anti-human sm22α (Abcam, Cambridge, MA) and anti-human β-actin (Abcam) antibodies. All signals detected by enhanced chemiluminescence.

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Huang L., Nakayama H., Klagsbrun M., Mulliken J.B, & Bischoff J. (2015). Glucose transporter 1-positive endothelial cells in infantile hemangioma exhibit features of facultative stem cells. Stem cells (Dayton, Ohio), 33(1), 133-145.

Publication 2014

anti- αTubulin anti-JAGGED1 anti-human CD31 anti-human sm22α anti-human β-actin

Actin Antibodies Calponin Cell extracts Chemiluminescence Human Jagged1 Nitrocellulose Sds page

Corresponding Organization : Harvard University

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Variable analysis

independent variables

Cell extracts were prepared [9 (link), 32 (link)]

dependent variables

Signals detected by enhanced chemiluminescence

control variables

Sodium dodecyl sulfate– polyacrylamide gel electrophoresis (SDS-PAGE)
Transferred to nitrocellulose
Probed with anti-human PlexinD (R&D Systems), anti- αTubulin (EMD Millipore), anti-JAGGED1(Cell Signaling Technology, Danvers, MA), anti-human Calponin (Dako, Carpinteria, CA), anti-human CD31 (Santa Cruz Biotechnology, Inc., Dallas, Texas), anti-human sm22α (Abcam, Cambridge, MA) and anti-human β-actin (Abcam) antibodies

controls

Positive controls: Not mentioned
Negative controls: Not mentioned

Annotations

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