Zebrafish used in this study was Tübingen strain that was initially obtained from the Zebrafish International Resource Center and propagated in our lab according to the following procedure. Fish were kept at constant water temperature (28°C), photoperiod (14L:10D, lights on 9:00, lights off at 23:00), pH (7.2), and salinity (conductivity 500–1200 μS) in automatic controlled zebrafish rearing systems (Aquatic Habitats Z-Hab Duo systems, FL, USA). Fish were fed three times daily to satiation with a commercial food (Otohime B2, Reed Mariculture, CA, USA) contained high protein content and supplemented with newly hatched artemia (Brine Shrimp Direct, UT, USA). Fertilized eggs were collected from natural spawning of paired healthy fish in the morning once lights were turned on. Experimental protocols were approved by The Institutional Animal Care and Use Committee at East Carolina University.
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