Protocol detail

Antidepressant Effects on Cell Proliferation

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LCLs were cultured in RPMI medium and incubated with antidepressants for 21 days as described elsewhere.^{16 (link)} Fluoxetine and citalopram (Sigma-Aldrich, Taufkirchen, Germany) stock solutions were prepared in dimethyl sulfoxide. Proliferation rates were measured using the EdU (5-ethynyl-2′-deoxyuridine) incorporation assays (Thermo Fisher Scientific, Darmstadt, Germany; catalog no. C10635) as reported before.^{16 (link)} Relative proliferation rates were calculated as ratio between treated and untreated samples of the same cell lines.

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Breitfeld J., Scholl C., Steffens M., Laje G, & Stingl J.C. (2017). Gene expression and proliferation biomarkers for antidepressant treatment resistance. Translational Psychiatry, 7(3), e1061-.

Publication 2017

Fluoxetine citalopram

5 ethynyl 2 deoxyuridine Antidepressants Assays Cell lines Citalopram Cultured medium Dimethyl sulfoxide Fluoxetine Lcls

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Variable analysis

independent variables

Fluoxetine
Citalopram

dependent variables

Proliferation rates

control variables

RPMI medium
Cell lines
Incubation duration (21 days)

controls

Positive control: Treated samples
Negative control: Untreated samples

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