UC-MSCs were obtained and isolated as previously reported (19 (link),20 (link)). Briefly, umbilical cords were collected from term infants immediately after birth. The cords were rinsed twice in phosphate-buffered saline (PBS) with 100 units/mL penicillin and streptomycin, and the cord blood vessels were carefully removed. Wharton’s jelly was cut into 1–2 mm3 pieces and resuspended in Dulbecco’s modified Eagle’s medium (DMEM)/F-12 containing 10% fetal bovine serum (FBS) (all from Gibco, Life Technologies). After culture for 2 days, the medium containing non-adherent cells was discarded, and the medium was replaced twice a week thereafter. MSCs between passages 4 to 6 were used for subsequent experiments.
Protocol detail
Isolation and Culture of UC-MSCs
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Birth
Cells
Cords
Eagle
Fetal bovine serum
Infants
Penicillin
Phosphate
Saline
Streptomycin
Umbilical cords
Wharton jelly
Corresponding Organization : Nanjing Drum Tower Hospital
Other organizations : Soochow University, First Affiliated Hospital of Soochow University
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Variable analysis
independent variables
- Isolation and culture of UC-MSCs
- Characteristics and properties of UC-MSCs
- Umbilical cords were collected from term infants immediately after birth
- The cords were rinsed twice in phosphate-buffered saline (PBS) with 100 units/mL penicillin and streptomycin
- The cord blood vessels were carefully removed
- Wharton's jelly was cut into 1–2 mm³ pieces and resuspended in Dulbecco's modified Eagle's medium (DMEM)/F-12 containing 10% fetal bovine serum (FBS)
- The medium containing non-adherent cells was discarded after 2 days, and the medium was replaced twice a week thereafter
- MSCs between passages 4 to 6 were used for subsequent experiments
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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