Extracellular supernatants were collected from DMSO, sorafenib and siRNA treated samples at various times post-infection. Infectious viral titers were determined by plaque assay on Vero cells (Baer and Kehn-Hall, 2014 (link)).
Intracellular RNA was extracted using the RNeasy Mini Kit (Qiagen) according to manufacturer's protocol. Extracellular RNA was extracted from supernatants using the MagMAX™−96 Viral RNA Isolation Kit (Life Technologies) according to manufacturer's protocol. Absolute quantification of RVFV genomic copies was determined by RT-quantitative (q)PCR as previously published (Shafagati et al., 2013 (link)).
Intracellular RNA was extracted using the RNeasy Mini Kit (Qiagen) according to manufacturer's protocol. Extracellular RNA was extracted from supernatants using the MagMAX™−96 Viral RNA Isolation Kit (Life Technologies) according to manufacturer's protocol. Absolute quantification of RVFV genomic copies was determined by RT-quantitative (q)PCR as previously published (Shafagati et al., 2013 (link)).
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