Blood Panel and Adipokine Assay Protocol

Details of the sample collection were described in a previous study [7 (link)]. After a fasting period of 12 h, venous blood specimens were collected and stored at − 70 °C until use in further analysis. Levels of the lipid panel, free fatty acids, a glucose-related panel, serum gamma-glutamyltransferase (gamma-GT) assayed via a modified Sanz method, and serum high-sensitivity C-reactive protein (hs-CRP) assayed via a latex-agglutination turbidimetric immunoassay were measured by a Hitachi 7600 autoanalyzer (Hitachi, Tokyo, Japan). Plasma adiponectin concentrations were measured by an enzyme immunoassay [11 (link)], and leptin was measured with a Human Leptin ELISA kit (Millipore, Darmstadt, Germany) by SpectraMax190 (Molecular Devices, Shanghai, China).

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Jang H.Y., Han Y., Yoo H.J., Lee J.H, & Kim M. (2021). Effects of short-term dietary restriction on plasma metabolites and the subcutaneous fat area according to metabolic status in obese individuals: a case–control study. Diabetology & Metabolic Syndrome, 13, 62.

Publication 2021

Hitachi 7600 autoanalyzer Human Leptin ELISA kit SpectraMax190

Adiponectin Devices Elisa Enzyme immunoassay Free fatty acids Gamma glutamyltransferase Glucose High sensitivity c reactive protein Human Latex Leptin Lipid Plasma Sample collection Serum Turbidimetric immunoassay Venous blood

Corresponding Organization : Hannam University

Other organizations : Yonsei University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Levels of the lipid panel
Free fatty acids
Glucose-related panel
Serum gamma-glutamyltransferase (gamma-GT) assayed via a modified Sanz method
Serum high-sensitivity C-reactive protein (hs-CRP) assayed via a latex-agglutination turbidimetric immunoassay
Plasma adiponectin concentrations
Leptin measured with a Human Leptin ELISA kit

control variables

Fasting period of 12 h before blood collection
Storage of blood specimens at − 70 °C until use in further analysis

controls

No positive or negative controls were explicitly mentioned in the input.

Annotations

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