Fractional IPV Immunization in Rats

Immunisation regime was based on similar studies performed investigating fractional IPV responses21 (link)32 (link)35 (link).Under ketamine hydrochloride (Ceva) and xylazine hydrochloride (Troy Laboratories) sedation, rats (n = 5) were immunised with 1 or 0.2 D-antigen units of IPV 2 by Nanopatch or IM injection, with an additional positive-control group receiving a full human dose of 8 D-antigen units IM. Two Nanopatches, each containing half the dose to be delivered were applied to each ventral ear pinnae using a proprietary applicator at a velocity 3.1 ms⁻¹ and kept in place for 2 minutes. IM injections were administered into the hind leg by a 29G needle. Rats received three doses by Nanopatch or IM injection, at 21-day intervals, with blood samples collected one day before vaccination and 21 days post final dose.

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Muller D.A., Pearson F.E., Fernando G.J., Agyei-Yeboah C., Owens N.S., Corrie S.R., Crichton M.L., Wei J.C., Weldon W.C., Oberste M.S., Young P.R, & Kendall M.A. (2016). Inactivated poliovirus type 2 vaccine delivered to rat skin via high density microprojection array elicits potent neutralising antibody responses. Scientific Reports, 6, 22094.

Publication 2016

ketamine hydrochloride xylazine hydrochloride

A a 1 Blood D antigen Ear pinnae Human Im injection Immunisation Ketamine hydrochloride Needle Rats Sedation Vaccination Xylazine hydrochloride

Corresponding Organization :

Other organizations : University of Queensland, Centers for Disease Control and Prevention, National Center for Immunization and Respiratory Diseases

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Variable analysis

independent variables

Immunisation regime (1 or 0.2 D-antigen units of IPV 2 by Nanopatch or IM injection)

dependent variables

Antibody response (measured via blood samples collected one day before vaccination and 21 days post final dose)

control variables

Anesthesia (ketamine hydrochloride and xylazine hydrochloride)
Animal model (rats, n = 5)
Vaccination schedule (three doses at 21-day intervals)
Nanopatch application (two Nanopatches, each containing half the dose, applied to each ventral ear pinnae at a velocity of 3.1 ms^-1 for 2 minutes)
IM injection (administered into the hind leg by a 29G needle)

positive control

Full human dose of 8 D-antigen units of IPV 2 administered by IM injection

negative control

Not explicitly mentioned

Annotations

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