Mitochondrial-specific assessment of specific redox species was performed as previously described (Case et al., 2016 (link)). Briefly, cells were stained with 1 μM MitoSOX Red ( O2 -sensitive mitochondrial-localized probe, Thermo Fisher Scientific #M36008, Waltham, MA, USA) for 30 min at 37°C. Cells were analyzed on a LSRII flow cytometer at 488/610 nm ex/em, and data analyzed using FlowJo software.
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