Results are given either as means ± SEM of the original values or as means of % of control±SEM. Results exceeding + /−2SDs were excluded. Statistical analysis was carried out either by two-way ANOVA or by repeated measures ANOVA as appropriate and indicated for each analysis, followed by post-hoc Fisher’s Least Significant Difference (LSD) test, using STATISTICA software version 13 (StatSoft, Tulsa, Oklahoma). The data met the assumptions of the tests, namely, normal distribution and the variance between the compared groups was similar. p ≤ 0.05 was considered statistically significant. As a rule of thumb, according to our years of experience, and as can be found in others’ recent reports in a high impact journal [52 (link), 53 (link)] a minimal number of animals or samples/group to achieve reproducibility is 5.
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