CCCH-type Znf gene cloning and expression

PvC3H72 (Phytozome accession no.: Pavir.J07041.1) was previously identified as a CCCH-type Znf family gene and classified in the clade-XIV as a stress-responsive gene [18 (link)]. The full length gene was amplified from the gDNA of a selected line ‘HR8’ from switchgrass lowland ecotype ‘Alamo’ [47 (link)], and its encoded amino acid sequence is shown in Additional file 3: Figure S2. The gene was cloned into the Gateway entry vector pENTR/D (Invtirogen, Carlsbad, CA). The gene was subcloned into p2GWF7 [48 (link)], pGBKT7 (Invitrogen) and pVT1629 [47 (link)] through LR reaction (Invitrogen).

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Xie Z., Lin W., Yu G., Cheng Q., Xu B, & Huang B. (2019). Improved cold tolerance in switchgrass by a novel CCCH-type zinc finger transcription factor gene, PvC3H72, associated with ICE1–CBF–COR regulon and ABA-responsive genes. Biotechnology for Biofuels, 12, 224.

Publication 2019

p2GWF7 pGBKT7 LR reaction

Amino acid sequence Ecotype Gene Switchgrass Vector

Corresponding Organization : Rutgers, The State University of New Jersey

Other organizations : Nanjing Agricultural University, Nanjing Forestry University

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Variable analysis

independent variables

The full length gene was amplified from the gDNA of a selected line 'HR8' from switchgrass lowland ecotype 'Alamo'

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the provided information.

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