Quantitative IHC Image Analysis

The current plugin named as IHC profiler, integrates options for quantitative analysis of digital IHC images stained for either cytoplasmic or nuclear proteins. Demonstration video to perform quantitative scoring analysis of the cytoplasmic stained sample (Movie S1) and that of the nuclear stained sample (Movie S2) can be found in supplementary data. IHC profiler can be freely downloaded from Sourceforge website (https://sourceforge.net/projects/ihcprofiler/). IHC profiler is currently compatible for use with Microsoft Windows operating system. Guidelines pertaining to the use of IHC profiler and embedding it to the Windows based ImageJ program can also be found in the package.

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Varghese F., Bukhari A.B., Malhotra R, & De A. (2014). IHC Profiler: An Open Source Plugin for the Quantitative Evaluation and Automated Scoring of Immunohistochemistry Images of Human Tissue Samples. PLoS ONE, 9(5), e96801.

Publication 2014

Cytoplasmic Nuclear proteins

Corresponding Organization :

Other organizations : Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Hospital

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Protocol cited in 36 other protocols

Variable analysis

independent variables

Cytoplasmic stained sample
Nuclear stained sample

dependent variables

Quantitative scoring analysis of the cytoplasmic stained sample
Quantitative scoring analysis of the nuclear stained sample

control variables

Not explicitly mentioned

controls

Positive control: Not mentioned
Negative control: Not mentioned

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