Colitis Induction and Therapeutic Interventions in Mice
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Corresponding Organization :
Other organizations : Sorbonne Université, AgroParisTech, Université Paris-Saclay, Microbiologie de l’alimentation au service de la santé, Iltoo Pharma (France), Inserm, Paris Cardiovascular Research Center, Université d'Orléans, Centre National de la Recherche Scientifique, Hôpital Saint-Antoine, Assistance Publique – Hôpitaux de Paris, Centre de Recherche Saint-Antoine, Hôpital Lariboisière, Broad Institute, Harvard University, Massachusetts Institute of Technology, Laboratoire des Biomolécules
Protocol cited in 4 other protocols
Variable analysis
- Dextran sulfate sodium (DSS) administration in drinking water
- Administration of 6-formylindolo(3,2-b)carbazole (Ficz)
- Administration of AHR antagonist CH223191 (AHR-)
- Administration of three bacteria strains: Lactobacillus murinus, Lactobacillus reuteri, and Lactobacillus taiwanensis
- Administration of fluconazole in drinking water
- Administration of mouse IL-22–Fc
- Body weight
- Blood in stool
- Stool consistency
- Severity of colitis (assessed using disease activity index)
- Injection of DMSO vehicle alone for Ficz and AHR- treatment groups
- Oral gavage with PBS for control mice receiving bacterial strains
- Administration of isotype control (IgG2a) for IL-22 treatment
- Positive control: Mice injected with DMSO vehicle alone for Ficz and AHR- treatment groups
- Negative control: Oral gavage with PBS for control mice receiving bacterial strains
- Negative control: Administration of isotype control (IgG2a) for IL-22 treatment
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