CORT was prepared as previously described (David et al., 2009 (link)). Specifically, 35 μg/mL (equivalent to 5 mg/kg/day) CORT (27840; Sigma-Aldrich, St. Louis, MO, USA) was dissolved in 0.45% 2-hydroxypropyl cyclodextrin (332593; Sigma-Aldrich) and delivered in lightproof bottles, available ad libitum in drinking water. Control mice received 0.45% β-cyclodextrin in drinking water (C4767; Sigma-Aldrich). DOX was provided in chow containing 200 mg/kg DOX (S3888; Bioserv, Flemington, NJ, USA) fed ad libitum throughout life. Control chow (S4207; Bioserv) was used when DOX was withdrawn from the diet.
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