Corticosterone and Doxycycline Treatment Protocol

CORT was prepared as previously described (David et al., 2009 (link)). Specifically, 35 μg/mL (equivalent to 5 mg/kg/day) CORT (27840; Sigma-Aldrich, St. Louis, MO, USA) was dissolved in 0.45% 2-hydroxypropyl cyclodextrin (332593; Sigma-Aldrich) and delivered in lightproof bottles, available ad libitum in drinking water. Control mice received 0.45% β-cyclodextrin in drinking water (C4767; Sigma-Aldrich). DOX was provided in chow containing 200 mg/kg DOX (S3888; Bioserv, Flemington, NJ, USA) fed ad libitum throughout life. Control chow (S4207; Bioserv) was used when DOX was withdrawn from the diet.

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Besnard A., Langberg T., Levinson S., Chu D., Vicidomini C., Scobie K.N., Dwork A.J., Arango V., Rosoklija G.B., Mann J.J., Hen R., Leonardo E.D., Boldrini M, & Sahay A. (2018). Targeting Kruppel-like Factor 9 in Excitatory Neurons Protects against Chronic Stress-Induced Impairments in Dendritic Spines and Fear Responses. Cell reports, 23(11), 3183-3196.

Publication 2018

2-hydroxypropyl cyclodextrin β-cyclodextrin C4767 S3888 S4207

Cort Cyclodextrin Diet Hydroxypropyl Mice

Corresponding Organization : Broad Institute

Other organizations : Columbia University Irving Medical Center, New York Psychoanalytic Society and Institute, Macedonian Academy of Sciences and Arts

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Variable analysis

independent variables

CORT concentration (35 μg/mL, equivalent to 5 mg/kg/day)
CORT vehicle (0.45% 2-hydroxypropyl cyclodextrin)
DOX concentration (200 mg/kg in chow)

dependent variables

Not explicitly mentioned

control variables

Control mice received 0.45% β-cyclodextrin in drinking water
Control chow (S4207; Bioserv) was used when DOX was withdrawn from the diet

controls

Positive control: Mice receiving CORT in drinking water
Negative control: Mice receiving 0.45% β-cyclodextrin in drinking water

Annotations

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