Polyclonal IgG Purification from Survivor Plasma

We obtained de-identified previously collected plasma samples from survivors of Argentine hemorrhagic fever from the immune plasma bank at the Instituto Nacional de Enfermedades Virales Humanas (INEVH), based in Pergamino, Argentina. Provision of samples was approved by the INEVH Ethics Committee and the Harvard University Faculty of Medicine Committee on Human Studies (identified as not involving human subjects under 45CFR46.102(f)). We purified polyclonal IgG from de-identified Argentine hemorrhagic fever survivor plasma samples and from CR1 plasma with Protein G Ultralink Resin (Thermo Fisher Scientific) as previously described.^{14 (link)}