Immunohistochemical Analysis of Tumor Markers

To assess the expression of proliferation-related protein (Ki67), apoptosis-related proteins (BAX, BAK, BIM, BCL-2, BCL-XL and MCL1) and metastasis-associated protein (MMP9) in tumor tissues, immunohistochemistry assay was conducted with the following antibodies as previously reported [27 (link)]. Anti-Ki67 (ab245113), anti-BAX (ab32503), anti-BAK (ab32371), anti-BIM (ab32158), anti-BCL-2 (ab32124), anti-BCL-XL (ab32370), anti-MCL1 (ab32087) and anti-MMP9 (ab76003) were purchased from Abcam.

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Xu G., Liu J., He J., He H., Su X, & Gui Q. (2022). LOC554202 contributes to chordoma progression by sponging miR-377-3p and up-regulating SMAD3. Anti-Cancer Drugs, 34(1), 15-28.

Publication 2022

ab245113 ab32158), ab32370 ab76003

Antibodies Apoptosis proteins Assay Bcl 2 Immunohistochemistry Mcl1 Metastasis Mmp9 Protein Tissues Tumor

Corresponding Organization :

Other organizations : University of South China

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Variable analysis

independent variables

Anti-Ki67 (ab245113)
Anti-BAX (ab32503)
Anti-BAK (ab32371)
Anti-BIM (ab32158)
Anti-BCL-2 (ab32124)
Anti-BCL-XL (ab32370)
Anti-MCL1 (ab32087)
Anti-MMP9 (ab76003)

dependent variables

Expression of proliferation-related protein (Ki67)
Expression of apoptosis-related proteins (BAX, BAK, BIM, BCL-2, BCL-XL, MCL1)
Expression of metastasis-associated protein (MMP9)

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the information provided.

Annotations

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