Detecting DNA Damage: γH2AX and 8-OHdG

DNA damage was detected with γH2AX [29 (link)] and 8-hydroxy-2-deoxyguanosine (8-OHdG) [9 (link)]. The γH2AX antibody [30 (link)] was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Subsequently, Alexa Fluor^®488-secondary antibody (Cell Signaling Technology) and 7AAD were added for double staining. Additionally, 8-OHdG was examined with the 8-OHdG-FITC antibody (Santa Cruz Biotechnology). Finally, the as-prepared cells were subjected to flow cytometry.

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Hsu Y.Y., Chuang Y.T., Yen C.Y., Cheng M.Y., Chen C.Y., Cheng Y.B, & Chang H.W. (2022). Methanol Extract of Clavularia inflata Exerts Apoptosis and DNA Damage to Oral Cancer Cells. Antioxidants, 11(9), 1777.

Publication 2022

γH2AX antibody Alexa Fluor®488-secondary antibody 8-OHdG-FITC antibody

7aad 8 ohdg Alexa fluor 488 Antibody Cells Dna damage Fitc Flow cytometry

Corresponding Organization : Kaohsiung Medical University Chung-Ho Memorial Hospital

Other organizations : Taipei Medical University, Chi Mei Medical Center, Tzu Hui Institute of Technology

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Variable analysis

independent variables

γH2AX antibody obtained from Santa Cruz Biotechnology
8-OHdG-FITC antibody obtained from Santa Cruz Biotechnology
Alexa Fluor®488-secondary antibody obtained from Cell Signaling Technology

dependent variables

DNA damage detected with γH2AX
DNA damage detected with 8-hydroxy-2-deoxyguanosine (8-OHdG)

control variables

Not explicitly mentioned

controls

Positive control: Not mentioned
Negative control: Not mentioned

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