Histological Analysis of Tibial Growth Plates

Stained transverse sections of tibiae were viewed by an eclipse E400 Nikon light microscopy with ×4, ×10, ×20, or ×40 objectives, using light filters. Images were taken by a high-resolution camera (Olympus DP 71) controlled by Cell A software (Olympus, Japan). The thickness of the entire GPs, the proliferative zone (PZ), and the hypertrophic zone (HZ) as well as the number of cells were measured using the Cell A software with a measuring tool feature. Measurements were performed on these sections from 4 different animals from each group. In each slide, 10 random locations throughout the GPs were selected and measured [32 (link),33 (link)].

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Shitrit-Tovli A., Sides R., Kalev-Altman R., Meilich D., Becker G., Penn S., Shahar R, & Ornan E.M. (2022). The Use of Post-Natal Skeleton Development as Sensitive Preclinical Model to Test the Quality of Alternative Protein Sources in the Diet. Nutrients, 14(18), 3769.

Publication 2022

eclipse E400 Nikon light microscopy Cell A software

Animals Cell Hypertrophic Light Light microscopy Tibiae

Corresponding Organization : Hebrew University of Jerusalem

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Variable analysis

independent variables

Objective magnification (×4, ×10, ×20, or ×40)

dependent variables

Thickness of the entire growth plate (GP)
Thickness of the proliferative zone (PZ)
Thickness of the hypertrophic zone (HZ)
Number of cells in the GPs

control variables

Stained transverse sections of tibiae
Eclipse E400 Nikon light microscopy
High-resolution camera (Olympus DP 71)
Cell A software (Olympus, Japan)
Measuring tool feature in Cell A software
10 random locations throughout the GPs were selected and measured
Measurements were performed on these sections from 4 different animals from each group

controls

No positive or negative controls were explicitly mentioned in the input.

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