PBMC Flow Cytometry Immunophenotyping

Freshly isolated PBMC were stained for flow cytometry as described²² (link) using antibodies to the following human surface antigens. Cells were then fixed and permeabilized with a Cytofix-Cytoperm fixation/permeabilization kit (BD) and stained with anti-survivin (91630, R&D Systems, Minneapolis, MN, USA) and isotype control (mouse IgG1κ, R&D Systems). The cells were collected in FACSCantoII flow cytometer (BD), and the data were analyzed with FlowJo software (BD, v.10.7) and fluorescence minus one controls.

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Erlandsson M.C., Andersson K.M., Oparina N.Y., Chandrasekaran V., Saghy T., Damdimopoulos A., Garcia-Bonete M.J., Einbeigi Z., Silfverswärd S.T., Pekna M., Katona G, & Bokarewa M.I. (2022). Survivin promotes a glycolytic switch in CD4+ T cells by suppressing the transcription of PFKFB3 in rheumatoid arthritis. iScience, 25(12), 105526.

Publication 2022

Cytofix-Cytoperm fixation/permeabilization kit anti-survivin FACSCantoII

Antibodies Cells Flow cytometry Fluorescence Human Isotype Mouse Surface antigens Survivin

Corresponding Organization :

Other organizations : Sahlgrenska University Hospital, University of Gothenburg, Karolinska Institutet, Södra Älvsborg Hospital

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Variable analysis

independent variables

Antibodies to the following human surface antigens

dependent variables

Survivin expression

control variables

Freshly isolated PBMC
Cells were fixed and permeabilized with a Cytofix-Cytoperm fixation/permeabilization kit (BD)
Isotype control (mouse IgG1κ, R&D Systems)

controls

Isotype control (mouse IgG1κ, R&D Systems)
Fluorescence minus one controls

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