Isolation and Transfer of ILC2 Precursors

Bones were cleaned, washed in 70% EtOH and subsequently in PBS and crushed using a pestle. Bones were rinsed with PBS and bone marrow was resuspended in PBS and filtered through a 70 μM cell strainer. Red blood cells were lysed using ACK buffer (Lonza) and lineage-positive cells were depleted using the Dynabeads untouched mouse CD4 cells kit according to the manufacture’s protocol (Thermo Fisher Scientific). Remaining cells were stained and ILC2ps were sort-purified as Lin⁻ Sca-1^high CD127⁺ CD25⁺ cells ^{14 (link)}. 1×10⁴ sort-purified ILC2ps were injected i.v. and mice were used at least 4 weeks after adoptive transfer.