Immunofluorescence Analysis of Stem Cell Markers

Immunofluorescence was performed as previously described^{10 (link)}. The following antibodies were used: β-Catenin mouse monoclonal (1:100), YAP1 mouse monoclonal (1:100), Runx2 mouse monoclonal (1:200), Osterix mouse monoclonal (1:500), VEGF-A mouse monoclonal (1:100), mouse IgG₁-FITC isotype control (1:200, Santa Cruz, CA, USA), β-Catenin rabbit polyclonal (1:100), CD31 rabbit monoclonal (1:500, Severicebio, Wuhan, China), Rabbit IgG monoclonal isotype control (1:200, Abcam, MA, USA), Alexa Fluor^® 647 conjugated Goat Anti-Mouse IgG (1:100), Alexa Fluor^® 488 conjugated Goat Anti-Mouse IgG (1:100, Abcam, MA, USA), Cy5 conjugated Goat Anti-rabbit IgG (1:100), Alexa Fluor^® 488-conjugated Goat Anti-Rabbit IgG (1:100), Cy3 conjugated Goat Anti-Rabbit IgG (1:100, Severicebio), and DAPI (Leagene, Beijing, China).