The capture areas were incubated with 60% DMSO (472301, Sigma-Aldrich) at RT for 5 min20 (link), after which they were washed three times with 0.1 × SSC buffer (S6639, Sigma-Aldrich). Important note: if the sRIN slide was stored dried prior to hybridization or de-hybridization, it was required to perform at short re-hydration step in 0.1 × SSC (S6639, Sigma-Aldrich) at RT for 5 min20 (link).
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