Protein Expression Analysis by Western Blot

Western blot analysis was performed on cell and tissue lysates as described.^{62 (link)} Antibodies used in this study were as follows: phospho-STAT3 (Y705) (no. 9145) and STAT3 (no. 4904) from Cell Signaling (Danvers, MA), Cyclin D1 (sc-717) from Santa Cruz Biotechnologies (Santa Cruz, CA), β-actin (A1978) from Sigma-Aldrich (St. Louis, MO, USA), and GAPDH (no. 3783) from ProsciΨ (Poway, California, USA).

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Hou S., Yi Y.W., Jin Kang H., Zhang L., Kim H.J., Kong Y., Liu Y., Wang K., Kong H.S., Grindrod S., Bae I, & Brown M.L. (2014). Novel Carbazole Inhibits Phospho-STAT3 through Induction of Protein–Tyrosine Phosphatase PTPN6. Journal of medicinal chemistry, 57(15), 6342-6353.

Publication 2014

STAT3 Cyclin D1 (sc-717) β-actin (A1978)

Actin Antibodies Cell Cyclin d1 Gapdh Stat3 Tissue Western blot analysis

Corresponding Organization :

Other organizations : Georgetown University Medical Center, Georgetown University

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Phospho-STAT3 (Y705)
STAT3
Cyclin D1
β-actin
GAPDH

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the provided information.

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