Measuring Muscle Contractile Properties

Extensor digitorum longus (EDL) and soleus (SOL) muscles were excised and analyzed for force-generating capacities in vivo, as previously described [51 (link),52 (link),53 (link)]. Muscles were mounted on a dual-mode muscle lever system (300B-LR; Aurora Scientific Inc., Aurora, ON, Canada) in a 0.38-mL bath filled with Krebs Ringer bicarbonate that was maintained at 25 °C. Contractile characteristics tested included the following: peak twitch force, peak isometric tetanic force, time-to-peak twitch force and twitch half-relaxation time, and the maximal rates of tetanic contraction and relaxation.

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Nichenko A.S., Sorensen J.R., Southern W.M., Qualls A.E., Schifino A.G., McFaline-Figueroa J., Blum J.E., Tehrani K.F., Yin H., Mortensen L.J., Thalacker-Mercer A.E., Greising S.M, & Call J.A. (2021). Lifelong Ulk1-Mediated Autophagy Deficiency in Muscle Induces Mitochondrial Dysfunction and Contractile Weakness. International Journal of Molecular Sciences, 22(4), 1937.

Publication 2021

300B-LR

Bath Bicarbonate Contractile Muscle Soleus muscles Tetanic

Corresponding Organization : University of Georgia

Other organizations : University of Minnesota, Cornell University, University of Alabama at Birmingham

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Variable analysis

independent variables

Excision and analysis of the extensor digitorum longus (EDL) and soleus (SOL) muscles

dependent variables

Peak twitch force
Peak isometric tetanic force
Time-to-peak twitch force
Twitch half-relaxation time
Maximal rates of tetanic contraction
Maximal rates of tetanic relaxation

control variables

Muscles were mounted on a dual-mode muscle lever system (300B-LR; Aurora Scientific Inc., Aurora, ON, Canada) in a 0.38-mL bath filled with Krebs Ringer bicarbonate that was maintained at 25 °C

controls

No positive or negative controls were explicitly mentioned in the provided information.

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