The DeadEndTM Fluorometric TUNEL System kit (Promega, USA) was used according to the manufacturer’s protocol to detect DNA fragmentation.
Briefly, the samples were fixed in buffered formaldehyde, washed twice in PBS, treated with 0.2% Triton X-100 in PBS, and incubated in an equilibration buffer.
Then, they were incubated in the reaction mix prepared according to the manufacturer’s instructions. The reaction was stopped by adding 2× saline-sodium citrate buffer and was
counterstained with Hoechst (0.1 mg/mL). The slides were evaluated using a fluorescent microscope.
Briefly, the samples were fixed in buffered formaldehyde, washed twice in PBS, treated with 0.2% Triton X-100 in PBS, and incubated in an equilibration buffer.
Then, they were incubated in the reaction mix prepared according to the manufacturer’s instructions. The reaction was stopped by adding 2× saline-sodium citrate buffer and was
counterstained with Hoechst (0.1 mg/mL). The slides were evaluated using a fluorescent microscope.
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