Dithizone Staining of Pancreatic Islets

Dithizone (Sigma-Aldrich, St Louis, MO, United States) is a zinc chelating agent, well-known to selectively stain pancreatic islets in a brownish red color. During islets purification and after 1 (T1), 14 (T14) and 28 days (T28) post-isolation, an aliquot of suspension of hPIs was stained with Dithizone to track the islet morphology during time and to observe the purity of preparation. 200 µL of Dithizone solution was added to 1 ml of islets suspension in culture media for 10 min at room temperature. Following incubation time, three washes in D-PBS 1X (Gibco) was performed and images were acquired using Zeiss light microscope at ×5 magnification.