Embryos were prepared for live imaging as previously described (Seleit et al., 2017a (link); Seleit et al., 2017b (link)). 1× Tricaine (Sigma-Aldrich #A5040-25G) was used to anaesthetize dechorionated medaka embryos (20 mg/ml – 20× stock solution diluted in 1XERM). Anaesthetized embryos were then mounted in low melting agarose (0.6–1%) (Biozyme Plaque Agarose #840101). Imaging was done on glass-bottomed dishes (MatTek Corporation Ashland, MA, USA). For g3bp1-eGFP live imaging, temperature was changed from 21 to 34°C after 1 hr of imaging.
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