Gene Expression Analysis of Cardiac Markers

Real-time RT-PCR was performed to analyze mRNA expression for Capn4, β-myosin heavy chain (β-MHC), atrial natriuretic peptide (ANP), collagen I and III, and GAPDH as described in our recent report (25 (link)).

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Li Y., Ma J., Zhu H., Singh M., Hill D., Greer P.A., Arnold J.M., Abel E.D, & Peng T. (2011). Targeted Inhibition of Calpain Reduces Myocardial Hypertrophy and Fibrosis in Mouse Models of Type 1 Diabetes. Diabetes, 60(11), 2985-2994.

Publication 2011

Atrial natriuretic peptide Collagen i Gapdh Mrna Myosin heavy chain Real time pcr

Corresponding Organization : Western University

Other organizations : Queen's University, University of Utah

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Variable analysis

dependent variables

MRNA expression for Capn4
MRNA expression for β-myosin heavy chain (β-MHC)
MRNA expression for atrial natriuretic peptide (ANP)
MRNA expression for collagen I
MRNA expression for collagen III
MRNA expression for GAPDH

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