High resolution ultrasound (US) imaging of normal and tumor mouse pancreas using the VisualSonics Vevo2100 High Resolution System with a VisualSonics MS-550D ultrasound transducer (35 MHz RMV)(VisualSonics, Inc., Toronto, ON, Canada) was performed as described [16 (link),17 (link)]. Then, 3D images were produced using VisualSonics Vevo2100 3D motor arm to collect serial images at 0.25 mm intervals through the entire thickness of the tumor. Tumors were outlined on each 2D image and reconstructed to quantify the 3D volume using the integrated Vevo2100 software package.
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