Antibody-Conjugated Quantum Dot Nanocarriers

The chemicals, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[18 (link)] (DSPE-PEG2000) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethylene glycol)2000] (DSPE-PEG2000-mal), were purchased from Avanti Polar Lipid, Inc. (Alabaster, AL, USA). Anti-EGFR antibody (cetuximab, Erbitux^®) was purchased from Merck (Darmstadt, Germany). QDs were purchased from ZEUS (Hwaseong, Republic of Korea). The PD-10 column and sepharose CL-4B were purchased from Amersham Bioscience (Uppsala, Sweden). Ultra-4 30 K MWCO was purchased from Amicon (Millipore, Darmstadt, Germany). Cell Counting kit-8 (CCK-8) was supplied by Dojindo Laboratories (Kumamoto, Japan). Doxorubicin hydrochloride was purchased from Merck (Darmstadt, Germany). Vyrant DiO Cell-Labeling Solution was purchased from Thermo Fisher Scientific (Waltham, MA, USA).