Serum Metabolite Quantification by HPLC-MS

One hundred fifty–microliter serum samples were spiked with calibration and internal standards and diluted with a 5% methanol solution containing 0.1% acetic acid. Samples were extracted using solid-phase extraction (HLB Oasis, Waters Chromatography) and analyzed using an high performance liquid chromotography system coupled to a 6460 Agilent Triple Quadrupole mass spectrometer (Agilent Technologies) [27 (link)].

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Schoeman J.C., Moutloatse G.P., Harms A.C., Vreeken R.J., Scherpbier H.J., Van Leeuwen L., Kuijpers T.W., Reinecke C.J., Berger R., Hankemeier T, & Bunders M.J. (2017). Fetal Metabolic Stress Disrupts Immune Homeostasis and Induces Proinflammatory Responses in Human Immunodeficiency Virus Type 1– and Combination Antiretroviral Therapy–Exposed Infants. The Journal of Infectious Diseases, 216(4), 436-446.

Publication 2017

6460 Agilent Triple Quadrupole mass spectrometer

Acetic acid Chromatography Methanol Oasis Serum Solid phase extraction

Corresponding Organization : Leibniz Institute of Virology (LIV)

Other organizations : Centre for Human Drug Research, Leiden University, North-West University

Top 5 similar protocols

Variable analysis

independent variables

Spiking of serum samples with calibration and internal standards
Dilution of samples with a 5% methanol solution containing 0.1% acetic acid

dependent variables

Analyte concentrations measured using high performance liquid chromatography coupled to a 6460 Agilent Triple Quadrupole mass spectrometer

control variables

Solid-phase extraction (HLB Oasis, Waters Chromatography)

controls

Calibration standards

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!