The cell lines SK-N-SH, and SH-SY5Y were purchased from Procell Life Science & Technology (CL-0214, CL-0208, Wuhan, China). SK-N-SH, SH-SY5Y, SK-N-BE(2) cells were cultured in MEM (C11095500BT, Gibco, NY, USA) containing 10% fetal bovine serum and an appropriate concentration of penicillin-streptomycin antibiotics (15140-122, Gibco, NY, USA). Cell line authentication was performed using short tandem repeat DNA profiling, and mycoplasma detection was regularly performed using a PCR-based method. All cell lines were cultured in a humidified atmosphere with 5% CO2 at 37°C.
Plasmids carrying CDC27 were constructed previously (17 (link)). Premixed siRNA was purchased from Santa Cruz (siCDC27:sc-77362, siNC: sc-37007). Growing cells were added to six-well plates and then transfected with plasmid or siRNA using Lipofectamine 2000 transfection reagent (Invitrogen) following the manufacturer’s instructions.
Plasmids carrying CDC27 were constructed previously (17 (link)). Premixed siRNA was purchased from Santa Cruz (siCDC27:sc-77362, siNC: sc-37007). Growing cells were added to six-well plates and then transfected with plasmid or siRNA using Lipofectamine 2000 transfection reagent (Invitrogen) following the manufacturer’s instructions.
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