Preparation and Characterization of DNA-Histone Complexes

Native and 8-oxodGuo containing oligonucleotides were purchased from
Shanghai Sangon Biotech Co. Ltd. dsDNAs (145 bp) were prepared by ligating short
oligonucleotides, as previously described^{40 (link)}. Expression and purification of mutant histones were
carried out according to reported protocols^{38 (link), 42 (link)}. Gels were
visualized using an Amersham Typhoon Gel and Blot Imaging System with excitation
and emission at 488 and 525 nm respectively.

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Bai J., Zhang Y., Xi Z., Greenberg M.M, & Zhou C. (2018). Oxidation of 8-oxo-7,8-dihydro-2’-deoxyguanosine leads to substantial DNA-histone cross-links within nucleosome core particles. Chemical research in toxicology, 31(12), 1364-1372.

Publication 2018

Typhoon Gel Blot Imaging System

8 oxodguo Histones Oligonucleotides Typhoon

Corresponding Organization : Collaborative Innovation Center of Chemical Science and Engineering Tianjin

Other organizations : Johns Hopkins University

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Variable analysis

independent variables

Native and 8-oxodGuo containing oligonucleotides
Mutant histones

dependent variables

Visualization of gels using Amersham Typhoon Gel and Blot Imaging System

control variables

Protocols for preparation of dsDNAs (145 bp) and expression and purification of mutant histones

controls

Positive controls not explicitly mentioned
Negative controls not explicitly mentioned

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