Protein Expression Analysis in Liver and HSCs

The expression levels of proteins in livers and mouse HSCs were analyzed as previously described [35 (link)]. Primary antibodies were as follows: anti-mouse Cav1 (Cat. No.610406, 1:1000, BD Biosciences), anti-mouse α-SMA (Cat. No.A5228, 1:1000, Sigma-Alorich; Shanghai, China), anti-mouse Collagen α1(I) (Cat. No.ab6308, 1:200, Abcam), anti-mouse Collagen α1(III) (Cat. No.ab7778, 1:200, Abcam), anti phospho-Smad2 (Cat. No.ab53100, 1:1000, Abcam), anti-Smad2 (Cat. No.ab33875, 1:1000, Abcam), anti-β-Actin (Cat. No.12262, 1:1000, Cell Signaling, Shanghai, China). Goat anti-mouse IgG labeled with HRP (Cat. No.ab6789, 1:2000, Abcam) was used as secondary antibodies.